Pseudomonas orientalis F9: A Potent Antagonist against Phytopathogens with Phytotoxic Effect in the Apple Flower

by Zengerer, Veronika; Schmid, Michael; Bieri, Marco; Müller, Denise C.; Remus-Emsermann, Mitja N. P.; Ahrens, Christian H. and Pelludat, Cosima
Abstract:
In light of public concerns over the use of pesticides and antibiotics in plant protection and the subsequent selection for spread of resistant bacteria in the environment, it is inevitable to broaden our knowledge about viable alternatives, such as natural antagonists and their mode of action. The genus Pseudomonas is known for its metabolic versatility and genetic plasticity, encompassing pathogens as well as antagonists. We characterized strain P. orientalis F9, an isolate from apple flowers in a Swiss orchard, and determined its antagonistic activity against several phytopathogenic bacteria, in particular Erwinia amylovora, the causal agent of fire blight. P. orientalis F9 displayed antagonistic activity against a broad suite of phytopathogenic bacteria in the in vitro tests. The promising results from this analysis led to an ex vivo assay with E. amylovora CFBP1430Rif and P. orientalis F9 infected detached apple flowers. F9 diminished the fire blight pathogen in the flowers but also revealed phytotoxic traits. The experimental results were discussed in light of the complete genome sequence of F9, which revealed the strain to carry phenazine genes. Phenazines are known to contribute to antagonistic activity of bacterial strains against soil pathogens. When tested in the cress assay with Pythium ultimum as pathogen F9 showed results comparable to the known antagonist P. protegens CHA0.
Reference:
Pseudomonas orientalis F9: A Potent Antagonist against Phytopathogens with Phytotoxic Effect in the Apple Flower (Zengerer, Veronika; Schmid, Michael; Bieri, Marco; Müller, Denise C.; Remus-Emsermann, Mitja N. P.; Ahrens, Christian H. and Pelludat, Cosima), In Frontiers in Microbiology, volume 9, 2018.
Bibtex Entry:
@Article{Zengerer2018,
  Title                    = {Pseudomonas orientalis F9: A Potent Antagonist against Phytopathogens with Phytotoxic Effect in the Apple Flower},
  Author                   = {Zengerer, Veronika and Schmid, Michael and Bieri, Marco and Müller, Denise C. and Remus-Emsermann, Mitja N. P. and Ahrens, Christian H. and Pelludat, Cosima},
  Journal                  = {Frontiers in Microbiology},
  Year                     = {2018},
  month                    = 2,   
  Pages                    = {145},
  Volume                   = {9},

  Abstract                 = {In light of public concerns over the use of pesticides and antibiotics in plant protection and the subsequent selection for spread of resistant bacteria in the environment, it is inevitable to broaden our knowledge about viable alternatives, such as natural antagonists and their mode of action. The genus Pseudomonas is known for its metabolic versatility and genetic plasticity, encompassing pathogens as well as antagonists. We characterized strain P. orientalis F9, an isolate from apple flowers in a Swiss orchard, and determined its antagonistic activity against several phytopathogenic bacteria, in particular Erwinia amylovora, the causal agent of fire blight. P. orientalis F9 displayed antagonistic activity against a broad suite of phytopathogenic bacteria in the in vitro tests. The promising results from this analysis led to an ex vivo assay with E. amylovora CFBP1430Rif and P. orientalis F9 infected detached apple flowers. F9 diminished the fire blight pathogen in the flowers but also revealed phytotoxic traits. The experimental results were discussed in light of the complete genome sequence of F9, which revealed the strain to carry phenazine genes. Phenazines are known to contribute to antagonistic activity of bacterial strains against soil pathogens. When tested in the cress assay with Pythium ultimum as pathogen F9 showed results comparable to the known antagonist P. protegens CHA0.},
  Doi                      = {10.3389/fmicb.2018.00145},
  ISSN                     = {1664-302X},
  Owner                    = {michael},
  Timestamp                = {2018.08.04},
  Url                      = {https://www.frontiersin.org/article/10.3389/fmicb.2018.00145}
}