We apply all relevant Next- and Third-generation Sequencing Technologies to get the best Results possible

Pacific Biosystems
(PacBio)

Long sequencing reads for contiguous and accurate assemblies

Pacific Biosystems® SMRT® Sequencing technology brought long-read sequencing to a level where high-quality and highly contiguous assemblies can be generated by relying solely on this technology.

genexa uses PacBio for the following key applications:

Complete and circular prokaryotic assemblies of high accuracy (> 99.999%)
Detection of small & large scale variants
De novo assembly of metagenomes from low to moderate complexity

Contact us if you want to establish PacBio sequencing technology in your lab.

Oxford Nanopore Technologies

Super long sequencing reads, tiny sequencer and a lot of potential

genexa uses Oxford Nanopore Technologies® for the following key applications:

Resolving very long genetic repeats as expected in some prokaryotes and in general in eukaryotes
De novo assembly of metagenomes from low to moderate complexity
Rapid taxonomic profiling of metagenomes
Rapid detection of specific organisms in your samples

Contact us if you want to establish nanopore sequencing technology in your lab.

Illumina sequencing

Short but highly accurate sequencing reads

Illumina® sequencing has been fully established in the genomics community for many years. Its "sequencing by synthesis" technology remains the "work horse" sequencing technology in genomics.

genexa uses Illumina sequencing for the following applications:

Draft assemblies of single genomes & metagenomes
Error correction of assemblies based on long-read sequencing
Small-scale variant detection
Taxonomic profiling via shotgun metagenomic sequencing

A quick comparison of all three sequencing technologies:

This comparison is only available on large screen devices!

	Pacific Biosystems	Oxford Nanopore Technologies	Illumina
Technology	SMRT® Sequencing (Sequel® and RS II® systems)	Nanopore sequencing (MinION®, GridION®, etc.)	MiSeq®, NextSeq®, etc.
Read length	Dozens of kilobases, up to about 200 kb	Dozens of kilobases, up to several hundred kb	100–300 bp (paired reads)
Key applications for genexa	Highly contiguous assemblies with very high accuracy, large-scale variant detection	Highly contiguous assemblies, rapid sequencing, large-scale variant detection	Deep sequencing, polishing, small-scale variant detection
Type / Underlying principle	Long-read / Sequencing by synthesis	Long-read / Nanopore sequencing	Short-read / Sequencing by synthesis
Accuracy of single reads	medium to high (70–99%, depending on mode of use)	medium to high (70–95% depending if 1D or 1D²)	very high (> 99%)
Website	pacb.com	nanoporetech.com	illumina.com