We apply all relevant Next- and Third-generation Sequencing Technologies to get the best Results possible

Pacific Biosystems

Long sequencing reads for contiguous and accurate assemblies

Pacific Biosystems® SMRT® Sequencing technology brought long-read sequencing to a level where high-quality and highly contiguous assemblies can be generated by relying solely on this technology.

Genexa uses PacBio for the following key applications:

  • Complete and circular prokaryotic assemblies of high accuracy (> 99.999%)
  • Detection of small & large scale variants
  • De novo assembly of metagenomes from low to moderate complexity

Contact us if you want to establish PacBio sequencing technology in your lab.

Oxford Nanopore Technologies

Super long sequencing reads, tiny sequencer and a lot of potential

Genexa uses Oxford Nanopore Technologies® for the following key applications:

  • Resolving very long genetic repeats as expected in some prokaryotes and in general in eukaryotes
  • De novo assembly of metagenomes from low to moderate complexity
  • Rapid taxonomic profiling of metagenomes
  • Rapid detection of specific organisms in your samples

Contact us if you want to establish nanopore sequencing technology in your lab.

Illumina sequencing

Short but highly accurate sequencing reads

Illumina® sequencing has been fully established in the genomics community for many years. Its "sequencing by synthesis" technology remains the "work horse" sequencing technology in genomics.

Genexa uses Illumina sequencing for the following applications:

  • Draft assemblies of single genomes & metagenomes
  • Error correction of assemblies based on long-read sequencing
  • Small-scale variant detection
  • Taxonomic profiling via shotgun metagenomic sequencing

A quick comparison of all three sequencing technologies:

This comparison is only available on large screen devices!

Pacific BiosystemsOxford Nanopore TechnologiesIllumina
TechnologySMRT® Sequencing (Sequel® and RS II® systems)Nanopore sequencing (MinION®, GridION®, etc.)MiSeq®, NextSeq®, etc.
Read lengthDozens of kilobases, up to about 200 kbDozens of kilobases, up to several hundred kb100–300 bp (paired reads)
Key applications for GenexaHighly contiguous assemblies with very high accuracy, large-scale variant detectionHighly contiguous assemblies, rapid sequencing, large-scale variant detectionDeep sequencing, polishing, small-scale variant detection
Type / Underlying principleLong-read / Sequencing by synthesisLong-read / Nanopore sequencingShort-read / Sequencing by synthesis
Accuracy of single readsmedium to high (70–99%, depending on mode of use)medium to high (70–95% depending if 1D or 1D²)very high (> 99%)

Additional technologies to perform optical genome mapping or to extract linkage information can be essential to solve your research question – We are happy to apply them to improve your analysis:

  • Bionano Genomics®: Get long-range genome mapping information and resolve large repeats and large-scale structural variations
  • 10x Genomics®: Get linkage information to improve metagenome assemblies or polyploid genomes
  • Hi-C: Pair your genetic elements (e.g. plasmids to chromosomes) in your metagenome sample